Journal: Macromolecular bioscience

Article Title: Patterned hydrogel substrates for cell culture with electrohydrodynamic jet printing.

doi: 10.1002/mabi.201100004

Figure Lengend Snippet: Figure 3. Droplets of IgG printed on 9.6 kPa (a) and 34.4 kPa (b) gels. Scale bars are 50 mm. Confocal image shows that IgG was located at the surface, but penetrated approximately 30 mm into a 21.1 kPa gel (c). Immunofluorescent detection of printed fibronectin on 21.1 kPa gels showing precise placement with spot sizes down to approximately 5 mm in diameter (d–f). Scale bars are 50 mm.

Article Snippet: Fibronectin was visualized after treatment with sheep anti-human fibronectin IgG (R&D Systems) and NL493-conjugated donkey anti-sheep IgG (R&D Systems).

Techniques:

Journal: Macromolecular bioscience

Article Title: Patterned hydrogel substrates for cell culture with electrohydrodynamic jet printing.

doi: 10.1002/mabi.201100004

Figure Lengend Snippet: Figure 4. A primary mesenchymal stem cell stained for filamen- tous actin (a) elongated and aligned with a fibronectin stripe (b) detected with immunofluorescence. A composite image is shown in (c). A phase contrast image of D1 cells adhered to equally spaced spots of fibronectin (d). Spots can be printed with a small enough diameter to constrain single cells. A phase contrast image of densely packed D1s on a narrow rectangle of patterned fibro- nectin (e). Scale bars are 50 mm.

Article Snippet: Fibronectin was visualized after treatment with sheep anti-human fibronectin IgG (R&D Systems) and NL493-conjugated donkey anti-sheep IgG (R&D Systems).

Techniques: Staining